Intestinal microbiota in neonates within three days after birth

• DOI: 10.3760/cma.j.issn.1007-9408.2017.07.006
• Publication Date: 2017-07-16
• Authors: Yifang Ding; Lingli Xiao; Jialin Guo; Jiong Lu; Hao Xu; Mei-Ling Hou; Ben Xiao-ming
• Keywords: Bacterial phyla

Objective To investigate the characteristics of intestinal microbiota in neonates on first and third day after birth.

Methods A total 50 healthy singleton who were born between June 15, 2016 August 3, Shanghai First Maternity Infant Hospital enrolled. Their stool samples collected birth labeled according to time collection (D1 D3 groups, n=50 each). Illumina NexSeq high-throughput sequencing platform was used sequence variable region 4 5 all bacterial 16S rRNA genes samples. The composition microbial communities determined differences two groups compared by Metastats analysis.

Results (1) A 100 sequenced retrieved sequences from 25 phyla, 119 families, 227 genera 159 species. (2) Major phyla same, namely, Proteobacteria, Frimicutes, Bacteroidetes Actinobacteria. relative abundances Frimicutes (0.27±0.03 vs 0.41±0.05) (0.07±0.01 0.09±0.03) increased over time, while that Actinobacteria (0.10±0.01 0.01±0.00) decreased 3. No significant difference abundance Proteobacteria (0.51±0.03 0.49±0.05) observed D1 groups. There (both q=-0.01, both P<0.05). (3) Among top ten most abundant Enterobacteriaceae, Staphylococcaceae, Enterococcaceae, Streptococcaceae Lachnospiraceae detected Enterobacteriaceae (0.25±0.02 0.46±0.06), Staphylococcaceae (0.07±0.02 0.12±0.03), Enterococcaceae (0.04±0.02 0.10±0.04), (0.03±0.02 0.06±0.01) (0.03±0.01 0.02±0.02) Only had statistical (q=0.00, (4) genera, Staphylococcus, Enterococcus, Streptococcus, Bacteroides Pseudomonas aerobic facultative anaerobic bacteria which belonged Stenotrophomonas, Propionibacterium, Acinetobacter, Bacillus, Sphingomonas so 3 as with those 1 (0.00±0.00 0.07±0.02, 0.00±0.00 0.06±0.01, 0.03±0.01, 0.02±0.01, 0.02±0.00, q=0.00, However, Bacteroides, Veillonella, Parabacteroides (0.01±0.00 0.08±0.03, 0.03±0.02, 0.01±0.00, q=0.01, 0.01 0.00, (5) species escherichia coli Three less including lactobacillus gasseri, animalis bifidobacterium bifidum (6) Regardless mode delivery, highest predominant meconium samples, followed stenotrophomonas. Stool divided into four based deliver modes feeding patterns. Neonates abdominally exclusive breastfed thereafter different other three bacteria, but not statistically significant. Bifidobacterium Subdoligranulum only vaginally neonates.

Conclusions Meconium is sterile. Although life life, dominant are common. During days significantly increased.

Key words: Gastrointestinal tract; Microbial consortia; High-throughput nucleotide sequencing; Infant, newborn