Expression of matrix metalloproteinases-9 in the brain tissue of experimental hepatic encephalopathy rats

• DOI: 10.3760/cma.j.issn.1008-6706.2015.02.006
• Publication Date: 2015-01-15
• Authors: Lihua Hu; Peng Chen; Xiaoli Wang; J. Z. Zhang; Yanjie Zhang
• Keywords: Hepatic Encephalopathy; Pathogenesis

Objective To investigate the significance of MMP-9 expression in pathogenesis hepatic encephalopathy.

Methods 180 rats were randomly divided into control group,hepatic encephalopathy 4,8 and 12h groups,45 each group.In group,rats intraperitoneally injected D-galactosamine(400mg/kg) with lipopolysaccharide(100μg/kg),and then symptom was observed.Ammonia,the evans blue(EB) content,the brain water content m RNA tissue assessed by real-time Q-PCR after 12h.

Results 2h later groups began to appear symptom.4 hours later,ammonia,the EB mRNA increase[(74.27±1.77)μmol/L vs(33.85±1.50)μmol/L,F= 5 983.36;(4.84±0.43)μg/g vs(3.96±0.48)μg/g,F= 111.54;(78.63±0.46)% vs(76.07±0.39)%,F= 737.25,and(1.037±0.071) vs(0.829±0.053),F= 132.10,P< 0.01],and extension time increased obviously[(136.32±3.88)μmol/L and(279.53±9.78)μmol/L,F= 983.36;(5.94±0.35)μg/ g and(6.71±0.50)μg/g,F= 111.54;(80.50±0.32)% and(82.14±0.30)%,F= 737.25;(1.235±0.095)and (1.425±0.113),F= 132.10,P<0.01].

Conclusion Blood barrier permeability enhancement edema may be related increasing.

Key words: Hepatic encephalopathy; Blood-brain barrier; Matrix metalloproteinases-9