Detection of porcine circovirus type 1 in African green monkey kidney cells

• DOI: 10.3760/cma.j.issn.1673-4211.2017.02.003
• Publication Date: 2017-04-10
• Authors: Yan Liu; Qingchuan Yu; Jialiang Du; Yueyue Liu; Dong Li; Jiamei Gao; Xingliang Fan; Yunqi Zhang; Rongrong Zhao
• Keywords: Vero cell; African Green Monkey; Agarose gel electrophoresis

Objective To develop detection methods for porcine circovirus type 1 (PCV1) — the adventitious agent in rotavirus vaccine contamination incident, African green monkey kidney cell (Vero cell).

Methods Six days after PCV1 infection, virus replication and proliferation Vero cells were tested with qualitative PCR, quantitative PCR (qPCR), electron microscopy (EM) situ hybridization (ISH).

Results PCR products amplified from PCV1-infected DNA extracts presented specific band agarose gel electrophoresis. According to qPCR, copy number increased 794.3 times of original level. The limits qPCR both 102.0 copies/ml. Characteristic viral particles observed by EM. ISH showed dark positive signal infected cells.

Conclusion The can be detected 4 used.

Key words: Circovirus; Vero cells; Polymerase chain reaction; Microscopy, electron; In