Research of characteristic chromatograms on Qihong decoction
Decoction
Chromatography detector
Gradient elution
Drift tube
DOI:
10.3760/cma.j.issn.1673-4246.2018.02.015
Publication Date:
2018-02-28
AUTHORS (6)
ABSTRACT
Objective
To develop an method for detecting the characteristic chromatograms of Qihong decoction by high-performance liquid chromatography coupled with diode arry and evaporative light-scattering detectors (HPLC-DAD-ELSD).
Methods
The determination was carried out Venusil MP-C18 (250 mm × 4.6 mm, 5 μm) column,using acetonitrile-0.2% formic acid as mobile phase gradient elution at a flow rate 1.0 ml/min detected wave length 254 nm, 290 365 nm. The drift tube temperature ELSD set 70 ℃, nebulizing gas 2.8 L/min.
Results
There were 34 chemical compositions in decoction. Among them, 16 peaks came from Polygoni Orientalis Fructus, 17 Astagali Radix, respectively. A total 18 constituents identified.
Conclusions
The simple, steady reliable which could be applied to quality control decoction.
Key words:
Astragalus membranaceus; Fructus polygoni orientalis; Qihong decoction; Characteristic
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