The role of connective tissue growth factor-integrin ανβ5 signal pathway in extracellular matrix deposition of pulmonary artery smooth muscle cells

• DOI: 10.3760/cma.j.issn.1673-4912.2015.03.002
• Publication Date: 2015-03-20
• Authors: Cuixia Lu; Jie Fu; Gang Li; Yu Hu; Peng Jia; Jian Zhao; Pengyuan Chen

Objective To explore the effects of integrin ανβ5 and extracellular regulated protein kinase (ERK1/2) on matrix deposition pulmonary artery smooth muscle cells (PASMC) induced by connective tissue growth factor (CTGF) in vitro, to investigate mechanisms CTGF-integrin signal pathway vascular remodeling hypertension.

Methods (1) PASMC SD rat were cultured cell purity was evaluated α-SMA immunohistochemical staining. The experiment divided into control group, CTGF group (CTGF 50 ng/ml), antibody ng/ml+ P1F6 10 μg/ml). (2) Real-time fluorescent quantitative RT-PCR used detect role mRNA expression CollagenⅠ-α1, Collagen Ⅲ-α1 Fibronectin-1 genes. (3) Ⅲ, ERK1/2 p-ERK1/2 detected western blot immunohistochemistry assay.

Results (1) determined be Compared with assay indicated that Ⅲ-α1, increased significantly treated (50 ng/ml) for 72 h (P<0.05), while inhibited these genes (P<0.05). Western promoted Ⅲ protein, but still more than those group. using assay, ratio decreased (P<0.05).

Conclusion Integrin mediates CTGF. signaling may mediate effect PASMC, which play an important hypertension, maybe new target prevent occurrence development remodeling.

Key words: Integrin; Connective factor; Pulmonary cell; Extracellular matrix; Extracellular kinase; Rat