Research on the difference of C/EBP-α gene on hepatocyte and hepatic stellate cells in the autophagy and apoptosis

• DOI: 10.3760/cma.j.issn.1674-4756.2016.22.009
• Publication Date: 2016-11-25
• Authors: Yao‐Li Chen; Lili Tao; Guangyin Yu
• Keywords: Hepatic stellate cell

Objective To investigate the difference of autophagy and apoptosis hepatocyte hepatic stellate cells inducted by CCAAT enhancer binding protein -α(C/EBP-α) vitro study.

Methods The adenovirus mediated C/EBP-α gene (Ad-C/EBP-α) were constructed, BRL-3A cell lines HSC-T6 infected with 50, 100, 200 MOI, situation was detected flow cytometry. The expression marker LC3Ⅰ/LC3Ⅱ application Western blot method. Then, differences in pathways 2 induced investigated.

Results Flow cytometry showed that line significant 50 MOI line, dose increased, rate further upward trend (P<0.05). There no change line. HSC-T6LC3Ⅰ/LC3Ⅱ from level significantly decreased, higher, decline greater, downward line.

Conclusions The has a dose-dependent inhibitory effect on cells, which apoptosis, but it hepatocytes.

Key words: Hepatocyte; Hepatic cell; CCAAT protein-α; Apoptosis; Autophagy