Protective effects of Baicalin on intestinal mucosa injury by inhibiting nuclear factor-κB expression in rats with partial common bile duct ligation

• DOI: 10.3760/cma.j.issn.2095-428x.2014.07.009
• Publication Date: 2014-04-05
• Authors: Xijie Liu; Wenyu Feng; Lei Geng; Teng Liu; Bufeng Zheng
• Keywords: Baicalin; Intestinal mucosa; Group A

Objective To investigate the effects of Baicalin on intestinal mucosal injury in rats with partial common bile duct ligation (PCBDL).

Methods Forty male Wistar were randomly divided into 4 groups equally: sham operation, PCBDL, PCBDL1 and PCBDL2.Rats PCBDL2 subjected to ligation.Baicalin [80 mg/(kg·d)]was fed group (for 2 weeks) group(for 3 weeks), while for other groups, 9 g/L saline same volume was fed.Ileum mucosa prepared microscopic examination.The observed scored.The level NF-κB mRNA expression by Fluorescent Situ Hybridization, protein determined immunohistochemistry.

Results 1.Compared PCBDL group(3.2±0.5), pathological severity scores significantly declined (F=21.120, P<0.01) (1.9±0.2) (1.5±0.3).2.Compared ope-ration group(0.066±0.006), group(0.107±0.011), group(0.098±0.010), group(0.155±0.012)presented up-regulation(F=76.8, P<0.01).3.Compared group, positive rates epithelium increased.

Conclusions It is suggested that exert protective partially inhibiting mRNA, down-regulating epithelial cells.

Key words: Partial ligation; Baicalin; Intestinal cells; Nuclear factor-κB; Nuclear factor-κB