Dynamic expression changes of microR-146a and microR-29a in neonatal rats with acute lung injury

• DOI: 10.3760/cma.j.issn.2095-428x.2014.22.019
• Publication Date: 2014-11-20
• Authors: Li Chen; Benqing Wu; Wenqing Chen; Hanrong Cheng; Huiying Tu; Xiaoli Yi; Rong Rong

Objective To observe the dynamic expression changes of microRNA(miR)-146a and miR-29 in neonatal rats with acute lung injury (ALI) induced by lipopolysaccharide (LPS), to explore their influence on ALI.

Methods Totally 80 newborn aged 7 days were randomly assigned into LPS group control group.Intraperitoneal injection endotoxin(5 mg/kg) was done establish rat model ALI group.Rats intraperitoneally injected 0.1 mL 9 g/L saline.Lung tissue blood specimens from taken at 6 hours, 1 day, 3 after treatment.Lung pathological observed, levels serum tumor necrosis factor-alpha (TNF- α) detected enzyme-linked immunosorbent assay.The relative expressions tissues miR-146a miR-29a determined reverse transcription-polymerase chain reaction.

Results The hematoxylin-eosin staining indicated group.Compared group, TNF- α showed high (P< 0.05), while significantly reduced 0.05).

Conclusions Differential have an important role ALI.The key targets may provide new for early clinical diagnosis treatment ALI, as well research development medicine.

Key words: Neonatal rat; Acute injury; MicroRNA-146a; MicroRNA-29a