[Screening type II alveolar epithelial cell apoptosis related microRNA].

• DOI: 10.3760/cma.j.issn.2095-4352.2013.09.010
• Publication Date: 2013-09-01
• Authors: Hui Ji; Miao Chen; Ming-jiang Qian; Li Kang; Guo-yue Liu; Song Qin
• Keywords: Apoptotic body

To screen type II alveolar epithelial cell (AECII) apoptosis related microRNA (miRNA) with gene chip technology, provide a new strategy for the prevention and treatment of hyperoxia-induced acute lung injury(HALI).AECII male Sprague-Dawley (SD) rats was primarily cultured 36 hours, then exposed to 0.5 mmol/L H2O2 establish model. Transmission electron microscope (TEM) used identify AECII observe morphology. Before after injury 2.5, 6, 12 24 fluorescence-activated sorting (FACS) employed detect rate. Additionally, technology real time polymerase chain reaction (RT-PCR) were verify miRNA respectively.Microvilli osmiophilic multilamellar body found under TEM, which characteristic structure AECII. This proved that cells After cytoplasmic retraction, chromatin condensation margination, microvilli disappearance could be TEM. Compared blank control group, rate significantly increased H2O2, gradually [the early before hours (9.43±1.02)%, (18.38±2.91)%, (28.57±1.18)%, (35.83±2.66)% (57.68±2.22)%, respectively, all P<0.05]. injury, at accompanied changed expression profiling, in had been screened, they rno-miR-449a-5p, rno-miR-34b/c-5p, rno-miR-200a/c-3p, rno-miR-146a-5p, rno-miR-141-3p, rno-miR-21-5p, rno-miR-375-3p, rno-miR-29b-3p, rno-miR-214-5p, rno-miR-210-5p rno-miR-214-3p,and results RT-PCR consistent results.Screened out miRNA, miR-34 family may play key role regulation. Rno-miR-21-5p an important anti-apoptotic