[Effects of hydrogen sulfide on mitochondria of lung in rats with ALI induced by lipopolysaccharide].

• DOI: 10.3760/cma.j.issn.2095-4352.2017.01.007
• Publication Date: 2017-01-01
• Authors: Quansheng Du; Meng Zhang; Guofeng Li; Chao Wang; Nan Zhang; Jianxin Zhang
• Keywords: Malondialdehyde; Thiobarbituric acid

To observe the effects of hydrogen sulfide (H2S) on structure and function mitochondria lung in rats with acute injury (ALI) induced by lipopolysaccharide (LPS).Forty healthy male Sprague-Dawley (SD) were randomly divided into control group, LPS low-, middle-, high-dose NaHS groups, 8 each group. The group given 5 mg/kg via sublingual vein, those groups challenged for 3 hours followed intraperitoneally injection 0.78, 1.56 3.12 respectively a volume 2 mL/kg. mL/kg normal saline vein. sacrificed at 6 after model reproduction, tissues harvested time. isolated differential centrifugation. ultra structures observed electron microscope. content malondialdehyde (MDA) was determined thiobarbituric acid method, activities superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), adenosine triphosphatase (ATPase) xanthine oxidase method. mitochondrial activity swelling multiskan spectrum.It shown transmission microscope that normal. rat cells swollen disrupted or disintegrated cristae, osmiophilic lamellar bodies had fused disappeared, rough endoplasmic reticulum degranulation phenomenon obvious damage slightly mitigated low-dose it significantly middle-dose groups. Compared MDA increased (nmol/mg: 26.30±1.45 vs. 11.16±1.20), SOD, GSH-Px, ATPase decreased [SOD (U/mg): 18.78±1.13 27.44±1.97, GSH-Px 63.91±1.99 128.15±3.47, 4.83±0.25 9.92±0.65]; as well (A value: 0.164±0.025 0.319±0.045), 0.182±0.012 0.273±0.023), all statistical differences (all P < 0.01). contents 21.89±1.23, 17.63±1.56, 12.19±1.30 26.30±1.45), GSH-PX, 20.13±0.85, 21.38±1.22, 24.05±1.56 18.78±1.13; 82.06±1.65, 101.45±2.14, 117.80±2.12 63.91±1.99; 5.34±0.23, 7.06±0.37, 8.78±0.44 4.83±0.25]; markedly 0.194±0.018, 0.230±0.032, 0.297±0.038 0.164±0.025), 0.195±0.008, 0.219±0.017, 0.249±0.018 0.182±0.012), 0.05). Moreover, protective effect showed dose-dependent manner.It could be concluded induce structural functional impairment ALI LPS, H2S have beneficial against decreasing lipid peroxidation level protecting cell function, is correlated dosage.