High Throughput Assay to Examine Egg-Laying Preferences of Individual <em>Drosophila melanogaster</em>

• DOI: 10.3791/53716
• Publication Date: 2016-03-25T00:00:15Z
• Authors: Bin Gou; Edward Zhu; Ruo He; Ulrich Stern; Chung-Hui Yang
• Keywords: 0301 basic medicine; 0303 health sciences; 03 medical and health sciences; Drosophila melanogaster; Oviposition; Decision Making; Models, Animal; Animals; Drosophila Proteins; Female

Recently, egg-laying preference of Drosophila has emerged as a genetically tractable model to study the neural basis simple decision-making processes. When selecting sites deposit their eggs, female flies are capable ranking relative attractiveness options and choosing "greater two goods." However, most assays not practical if one wants take systematic genetic screening approach search for circuit underlying this process, they population-based laborious set up. To increase throughput studying preferences single females, we developed custom chambers that each can simultaneously assay up thirty individual well protocol ensures high rate (so is readily discernable more convincing). Our execute produces very consistent results. Additionally, these be equipped with different attachments allow video recording animals deliver light optogenetics studies. This article provides blueprints fabricating procedure preparing assayed in chambers.