Human iPSC-Derived Cardiomyocyte Networks on Multiwell Micro-electrode Arrays for Recurrent Action Potential Recordings

• DOI: 10.3791/59906-v
• Publication Date: 2019-07-16T00:00:20Z
• Authors: Viviana Zlochiver; Stacie L. Kroboth; Christopher R. Beal; Jonathan A. Cook; Rosy Joshi-Mukherjee
• Keywords: Cardiac Electrophysiology; Multielectrode array

Cardiac safety screening is of paramount importance for drug discovery and therapeutics. Therefore, the development novel high-throughput electrophysiological approaches hiPSC-derived cardiomyocyte (hiPSC-CM) preparations much needed efficient testing. Although multielectrode arrays (MEAs) are frequently employed field potential measurements excitable cells, a recent publication by Joshi-Mukherjee colleagues described validated its application recurrent action (AP) recordings from same hiPSC-CM preparation over days. The aim here to provide detailed step-by-step methods seeding CMs measuring AP waveforms via electroporation with high precision temporal resolution 1 µs. This approach addresses lack easy-to-use methodology gain intracellular access reliable investigations. A work flow plating hiPSC-CMs on multiwell MEA plates discussed emphasizing critical steps wherever relevant. In addition, custom-built MATLAB script rapid data handling, extraction analysis reported comprehensive investigation waveform quantify subtle differences in morphology various duration parameters implicated arrhythmia cardiotoxicity.