Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) clones from Paraguayan children

• DOI: 10.3855/jidc.12108
• Publication Date: 2020-04-01T11:37:36Z
• Authors: Fátima Rodriguez; Claudia Salinas; Silvina Fernandez; Sol Haim; Marta Mollerach; Wilma Basualdo; Hector Castro; Beatriz Quiñónez; Rocio Arguello; Monica Rodriguez; Lorena Grau; Carmen Espínola; Gladys Velázquez; Gloria Samudio; Gloria Gomez; Ana Campuzano; Juana Ortellado; Patricia Almada; Rosa Guillén
• Keywords: Molecular Epidemiology; Phage typing; Meticillin

Introduction: Staphylococcus aureus is considered one of the most important human pathogens, and its levels resistance to methicillin have increased even in strains isolated from people without nosocomial risk factors. Molecular analysis essential for understanding patterns dissemination. The objective this study was identify community-acquired methicillin-resistant S. (CA-MRSA) clones that infected Paraguayan children patients two periods time.
 Methodology: An observational, descriptive designed determine genetic variability 115 isolates CA-MRSA recovered who attended four reference centers Paraguay between 2009-2010 2012-2013.
 Results: combined use Pulsed Field Gel Electrophoresis (PFGE), Multi-Locus Sequencing Typing, Variable Analysis (MLVA) Spa typing techniques allowed identification dominant clones: ST30-IV-t019 (77%) ST5-IV-t311 (10%), establishment former as leading cause infections during period.
 Conclusions: This first provides epidemiological information well microbiological molecular characteristics Asunción Central Department Paraguay.