Comparison of P1 and 16S rRNA genes for detection of Mycoplasma pneumoniae by nested PCR in adults in Zhejiang, China

• DOI: 10.3855/jidc.5149
• Publication Date: 2015-03-15T14:43:42Z
• Authors: Zibo Zhou; Xiangzhi Li; Xiaojian Chen; Lili Yao; Changwang Pan; Huicong Huang; Fangjun Luo; Xiaoping Zheng; Xiaojing Sun; Feng Tan
• Keywords: Mycoplasma genitalium

Introduction: Mycoplasma pneumoniae (M. pneumoniae) is the most common atypical pathogen that causes respiratory infections in humans. Laboratory tests are important diagnosis of M. because features clinical signs and symptoms. Nowadays, both P1 adhesin gene 16S ribosomal (r) RNA (rRNA) have been widely detected by polymerase chain reaction (PCR). The purpose present study was to evaluate suitable target detection pneumonia via nested PCR. Methodology: Both rRNA for PCR conditions were optimized through an orthogonal test single-factor experiment. Then, sensitivity two sets targets evaluated. Finally, based on optimal conditions, 55 samples throat swabs collected from adult patients 2013 examined established Result: results revealed more sensitive than limits at least 100 fg 10 DNA, respectively. Furthermore, positive rate (30/55; 54.5%) higher (25/55; 45.5%). Conclusion: Our indicate infection due its samples.