Proteomic characterization of vitellogenins from three species of South American fresh water fish

• DOI: 10.3856/vol46-issue1-fulltext-18
• Publication Date: 2018-03-26T16:17:15Z
• Authors: Veronica Urdaneta; Emilio Camafeita; German Poleo; Hilda Guerrero; Carolina Bernal; Ivan Galindo; Nardy Diez
• Keywords: Vitellogenins; Marine fish

Vitellogenins (Vtg) are glycolipophosphoproteins synthesized by oviparous vertebrates as yolk proteins precursors.These have been studied for their role in reproduction and endocrine disruption.In this work, we report the first proteomic study towards characterization of Vtg from Pseudoplatystoma fasciatum, Piaractus brachypomus Colossoma macropomum.Male specimens each three fish species were estradiol-induced (experimental) non-induced (control).The initial was made 2D protein gel electrophoresis both groups The identification high molecular weight spots, presumed to be Vtgs, assessed MALDI-TOF mass spectrometry analysis.A post-translational modification performed differential staining gels order visualize phosphoproteins glycoproteins.Plasma samples species, induced with estrogen, showed spots variable isoelectric points.Post-translational modifications that Vtgs P. C. macropomum presented a phosphorylated glycosylated subunit, while same subunit fasciatum only glycosylated.This will help development reliable immunoassays, which could used studies disruption or improvement artificial spawning, uncovering time maturation sex determination.