influence of fibrinogen and laminin on bone mesenchymal stem cells osteogenic differentiation in pegda scaffold

• DOI: 10.3881/j.issn.1000-503x.2013.03.005
• Publication Date: 2013-06-01
• Authors: Zhi Zhan; Yin Liu; Jing Tian
• Keywords: Tissue Scaffolds; Osteogenesis; Animals; Fibrinogen; Bone Marrow Cells; Cell Differentiation; Mesenchymal Stem Cells; Laminin; Rabbits; Cells, Cultured

To explore the effects of fibrinogen(FG) and laminin(LN) in promoting the osteogenic differentiation of bone mesenchymal stem cells(BMSCs)in PEGDA scaffold.After the rabbit BMSCs were isolated and cultured to passage 3. BMSCs were blended in PEGDA-FG or PEGDA-LN hydrogels and cultured for 7 days. The levels of osterix,osteopontin,osteocalcin,collagen 2,myocardin,PPARΓ,and integrins Α2,Α5,and Α6 in PEGDA-FG and PEGDA-LN constructs were determined. Immunohistochemistry was used to detect the expressions of myocardin,PPARΓ,and OPN in PEGDA-FG and PEGDA-LN constructs.The expressions of osterix,OPN,and OC were significantly higher in PEGDA-FG scaffold than day 0(all P<0.05). The OPN and OC expression levels were significantly higher in PEGDA-LN scaffold than day 0(both P<0.05). In PEGDA-FG and PEGDA-LN scaffold,myocardin,PPARΓ and COL 2 expression level showed no significant differences than day 0(all P>0.05). Integrin Α2 was upregulated in PEGDA-LN scaffold than day 0(P<0.05). Integrin Α6 was upregulated in PEGDA-FG scaffold than day 0(P<0.05). Immunohistochemistry stain showed that OPN expression increased in PEGDA-FG and PEGDA-LN scaffolds.FG and LN can promote rabbit BMSCs osteogenic differentiation in PEGDA three-dimensional scaffold.