Identification of potential therapeutic target genes in mouse mesangial cells associated with diabetic nephropathy using bioinformatics analysis

• DOI: 10.3892/etm.2019.7524
• Publication Date: 2019-04-23T12:44:07Z
• Authors: Xin Mou; Di Zhou; Ying Liu; Kaiyuan Liu; Danyang Zhou
• Keywords: Identification; Mesangial cell

The aim of the present study was to identify genes under effect transforming growth factor‑β (TGF‑β1), high glucose (HG) and glucosamine (GlcN) in MES‑13 mesangial cells elucidate molecular mechanisms diabetic nephropathy (DN). gene expression datasets GSE2557 GSE2558 were downloaded from Gene Expression Omnibus database. Differentially expressed (DEGs) independently screened using GEO2R online tool. Ontology Kyoto Encyclopedia Genes Genomes (KEGG) pathway enrichment analyses performed Database for Annotation, Visualization, Integrated Discovery. protein‑protein interaction (PPI) network constructed Search Tool Retrieval Interacting Cytoscape software. hub identified by NetworkAnalyzer plugin. Overlapping subjected docking analysis SystemsDock. A total 202 upregulated 158 downregulated DEGs HG‑treated groups, 138 103 GlcN‑treated 81 44 TGF‑β1‑treated groups identified. majority enriched ‘nucleosome assembly’, ‘chromatin silencing’ ‘xenobiotic glucuronidation’. In addition, KEGG pathways significantly ‘systemic lupus erythematosus’, ‘protein processing endoplasmic reticulum’ ‘aldarate metabolism pathway’, ‘TNF signaling pathway’ intersected groups. total, eight genes, Jun, prostaglandin‑endoperoxide synthase 2 (Ptgs2), fibronectin 1 (Fn1), cyclin‑dependent kinase (Cdk)2, Fos, heat shock protein family (Hsp70) member 5 (Hspa5), Hsp90b1 homo sapiens hypoxia (Hyou1), three overlapping Ras homolog family, B (RHOB), complement factor H (CFH) Krüppel‑like 15 (KLF15), selected. Valsartan with RHOB, fosinopril CFH KLF15 had preferential binding activity. conclusion, Ptgs2, Fn1, Cdk2, Hspa5, Hsp90b1, Hyou1, may be potential therapeutic targets associated DN, which provide insight into DN treatment strategies.