Abnormal activation of complement C3 in the spinal dorsal horn is closely associated with progression of neuropathic pain

• DOI: 10.3892/ijmm.2013.1344
• Publication Date: 2013-04-11T10:53:25Z
• Authors: FACHUAN NIE; JINBAO WANG; DONG SU; YING SHI; JINMEI CHEN; HAIHUI WANG; WANXIANG QIN; LIN SHI
• Keywords: ATF3

The aim of the present study was to investigate role complement activation in pathogenesis neuropathic pain (NPP) induced by peripheral nerve injury. We modified a classical chronic constriction injury (CCI) model (mCCI), and verified its reliability rats. Furthermore, reverse transcription-PCR immunohistochemistry were conducted spinal dorsal horn effect inhibitor, cobra venom factor (CVF), on behavior mCCI found that rats group presented better general condition, without signs autophagy toes. Moreover, significant increase (+40%) expression component 3 (C3) mRNA horn, which associated with hyperalgesia. Correlation analysis showed negative correlation between mechanical threshold C3 cord. Administration CVF reduced occurrence hyperalgesia nearly reversed In addition, exhibited significantly less superoxide dismutase activity greater levels maleic dialdehyde compared sham-operated Transmission electron micrographs revealed mitochondrial swelling, cell membrane damage, cristae fragmentation neurons 14 days after mCCI. Mitochondrial swelling attenuated receiving CVF. findings demonstrated abnormal occurred cord NPP, could play an important cascade reaction complements are involved development