Hepatic differentiation of human iPSCs in different 3D models: A comparative study

• DOI: 10.3892/ijmm.2017.3190
• Publication Date: 2017-10-16T06:18:47Z
• Authors: Florian Meier; Nora Freyer; Joanna Brzeszczynska; Fanny Kn�spel; Lyle Armstrong; Majlinda Lako; Selina Greuel; Georg Damm; Eva Ludwig-Schwel...; Ulrich Deschl; James Ross; Mario Beilmann; Katrin Zeilinger
• Keywords: 3D cell culture; Regenerative Medicine

Human induced pluripotent stem cells (hiPSCs) are a promising source from which to derive distinct somatic cell types for in vitro or clinical use. Existent protocols hepatic differentiation of hiPSCs primarily based on 2D cultivation the cells. In present study, authors investigated generation hiPSC-derived hepatocyte-like using two different 3D culture systems: A scaffold-free microspheroid system and hollow-fiber perfusion bioreactor. The outcome these systems was compared with that conventional cultures, primary human hepatocytes as control. evaluation made specific mRNA expression, protein secretion, antigen expression metabolic activity. α-fetoprotein lower, while cytochrome P450 1A2 3A4 activities were higher system. Cells differentiated bioreactor showed an increased albumin hepatocyte nuclear factor 4α, well secretion α-1-antitrypsin system, suggesting degree maturation. contrast, provides easy robust method generate spheroids defined size screening applications, model instrument complex investigations under physiological-like conditions. conclusion, study introduces derived each demonstrating advantages individual applications benefits comparison cultures.