Effect and mechanism of propranolol on promoting osteogenic differentiation and early implant osseointegration

• DOI: 10.3892/ijmm.2021.5024
• Publication Date: 2021-08-19T08:39:39Z
• Authors: Yupeng Wu; Qi Zhang; Baodong Zhao; Xiaojing Wang
• Keywords: RUNX2

The present study aimed to investigate the effect of β‑receptor blocker propranolol on early osseointegration pure titanium implants and underlying molecular regulatory mechanisms. An implant model using tibial metaphysis New Zealand rabbits was established. were divided into control low‑, medium‑ high‑dose groups. formation detected by X‑ray scanning. Mesenchymal stem cells (MSCs) osteoblasts (OBs) isolated cultured in vitro, isoproterenol supplemented simulate sympathetic action subsequently administrated. cell proliferation osteogenic differentiation assessed EdU, flow cytometry, alizarin red staining alkaline phosphatase (ALP) detection. expression levels bone morphogenetic protein (BMP)2, RUNX family transcription factor (RunX)2, collagen (COL)‑1, osteocalcin (OCN) β2‑adrenergic receptor (AR) immunofluorescence, reverse transcription‑quantitative PCR western blot assay. Propranolol effectively promoted vivo, facilitated OBs, inhibited MSCs enhanced OBs MSCs. calcium content ALP activity treated with markedly higher than group. also elevated mRNA BMP2, RunX2, COL‑1 OCN tissue cells, decreased β2‑AR. demonstrated that osseointegration. provided a novel insight application mechanisms propranolol.