E2F1/SP3/STAT6 axis is required for IL-4-induced epithelial-mesenchymal transition of colorectal cancer cells

• DOI: 10.3892/ijo.2018.4429
• Publication Date: 2018-06-05T08:41:57Z
• Authors: Jiaoe Chen; Chaoju Gong; Huiqin Mao; Zhaoyun Li; Zejun Fang; Qiang Chen; Min Lin; Xiang Jiang; Yanyan Hu; Wei Wang; Xiaomin Zhang; Xianjun Chen; Hongzhang Li
• Keywords: E2F1; STAT6

Colorectal cancer (CRC) is a type of with mortality rate among the highest worldwide owing to its high metastasis. Therefore, inflammation-associated metastasis in development CRC currently topic considerable interest. In present study, pro-inflammatory cytokine interleukin-4 (IL-4) was identified promote epithelial-mesenchymal transition (EMT) cells. However, enhancing effect IL-4 more evident HCT116 cells compared RKO Accordingly, an increased expression level STAT6 observed Further investigations that E2F1 required for maintaining signal transducer and activator transcription 6 (STAT6) Mechanistically, induced specificity protein 3 (SP3) directly by binding promoter gene activating As result, phosphorylation-activated several EMT drivers, including zinc finger E-box-binding homeobox (Zeb)1 Zeb2, which serve critical function IL-4-induced EMT. Rescue experiments further confirmed relied on intact E2F1/SP3/STAT6 axis Finally, analysis clinical specimens revealed positive correlation between E2F1, SP3 STAT6. The ectopically expressed indicated poor prognosis patients CRC. conclusion, pathway be essential signaling-induced aggressiveness