Over-expression of bone sialoprotein enhances bone metastasis of human breast cancer cells in a mouse model

• DOI: 10.3892/ijo.23.4.1043
• Publication Date: 2014-03-10T03:29:14Z
• Authors: Jian-Hua Zhang; Jean Tang; Jie Wang; Wenli Ma; Wenling Zheng; Toshiyuki Yoneda; Jake Chen
• Keywords: Bone sialoprotein

Bone sialoprotein (BSP) is a major non-collagenous protein found almost exclusively in bone and other mineralized tissues including enamel, dentin cementum. Although role for BSP mineralization has been indicated, also appears to function patho-physiological processes, the metastasis of breast prostate cancer cells bone. The purpose this study was determine homing provide insights into physiological as well pathological processes. We established cultures MDA-231 stably transfected with DNA constructs pIRES2-EGFP (green fluorescent protein) expressing human (hBSP) cDNA (231BSP) under CMV promoter, or an antisense sequence hBSP (231BSPAS), empty vector control (231EV). These 3 cell groups were selected neomycin resistance using G418 analyzed by flow cytometry GFP expression. resultant cultured expressed different levels detected RT-PCR Western blot. Among three, 231BSP highest while 231BSPAS lowest. capacity tumor metastasize determined nude mice (5 each group) intra-cardiac injection from groups. Four weeks after inoculation, radiological examination revealed that all 5 group had developed osteolytic metastases. In only 1 mouse demonstrated metastatic lesions out (231EV) results strongly suggest over-expression can enhance whereas repressed expression BSP, cDNA, inhibits effect model.