Estrogen stimulates osteoprotegerin expression via the suppression of miR-145 expression in MG-63 cells

• DOI: 10.3892/mmr.2017.6168
• Publication Date: 2017-02-06T06:50:02Z
• Authors: Jun Jia; Hengxing Zhou; Xiantie Zeng; Shiqing Feng
• Keywords: Estrogen receptor alpha; Estrogen receptor beta

Osteoprotegerin (OPG) is implicated in the pathogenesis of postmenopausal osteoporosis, and other metabolic bone diseases caused by estrogen deficiency. Previous studies have demonstrated that may stimulate OPG expression osteoblast cells at transcriptional level; however, whether can regulate a post-transcriptional level remains elusive. The present study aimed to investigate role microRNA (miRNA) estrogen‑mediated production human osteoblast‑like MG‑63 cells. results from ELISA, western blotting reverse transcription-quantitative polymerase chain reaction (RT‑qPCR) confirmed upregulate expression. Mechanistic indicated increased activity luciferase reporter harboring 3'‑untranslated region (3'‑UTR). Bioinformatics analysis there potential targeting site 3'‑UTR for miRNA (miR)‑145, which associated with differentiation. an RT‑qPCR suggested suppressed miR‑145 In addition, dual‑luciferase assay, blot directly targets negatively regulates Furthermore, transfection mimics was able partially inhibit induction estrogen, thus confirming induction. Taken together, post-transcriptionally through suppression miR-145