Microarray and bioinformatics analyses of gene expression profiles in BALB/c murine macrophage polarization

• DOI: 10.3892/mmr.2017.7511
• Publication Date: 2017-09-19T03:53:19Z
• Authors: Li Jiang; Xueqin Li; Yingying Zhang; Mengying Zhang; Zongsheng Tang; Kun Lv
• Keywords: KEGG; Macrophage polarization

Macrophages possess the hallmark feature of plasticity, allowing them to undergo a dynamic transition between M1 and M2 polarized phenotypes. The aim present study was screen for differentially-expressed genes (DEGs) that were associated with BALB/c murine macrophage polarization. transcription profiles three samples obtained using microarray analysis. Based on threshold fold‑change >2.0 P‑value <0.05, total 1,253 DEGs identified, which 696 upregulated 557 downregulated in macrophages compared macrophages. Gene Ontology (GO) function Kyoto Encyclopedia Genes Genomes (KEGG) pathway enrichment analyses performed. A gene‑gene interaction network constructed Search Tool Retrieval Interacting database. GO annotation identified categories: Cellular component, molecular biological process, 34 40 terms consisting DEGs, respectively. analysis primarily protein binding, response stimulus, cell differentiation, regulation process. KEGG 15 four pathways involving Signaling revealed these mainly involved apoptosis, hypoxia‑inducible factor (HIF) 1a pathway, innate immune system, tumor necrosis (TNF) signaling cytokine‑cytokine receptor interaction, other signal transduction pathways. Interaction indicated including TNF, interleukin (IL)‑6, IL‑1β, suppressor cytokine 3, nitric oxide synthase 2, HIF1a may serve key roles provided new insights into role differentiation