Integrin β2 regulates titanium particle‑induced inflammation in macrophages: In vitro aseptic loosening model

• DOI: 10.3892/mmr.2024.13390
• Publication Date: 2024-11-11T13:45:56Z
• Authors: Yue Shen; Haruna Nakajima; Junfeng Zhu; Weigang Wu
• Keywords: Osteolysis

Aseptic loosening is a major complication of joint replacement surgery, characterized by periprosthetic osteolysis and chronic inflammation at the bone‑implant interface. Cells release chemokines, cytokines other pro‑inflammatory substances that perpetuate reactions, while particle‑stimulated macrophages promote osteoclastic bone resorption impair formation. The present study investigated integrin inflammatory cytokine expression patterns in RAW 264.7 cells treated with titanium (Ti) particles to elucidate role integrins Ti particle‑mediated osteolysis. Assessment was performed reverse transcription‑quantitative PCR, western blotting, confocal immunofluorescence, flow cytometry enzyme‑linked immunosorbent assays. Cell migration evaluated wound healing assay. It found significantly induced cells, including upregulation β2 (CD18), aL (CD11a), aM (CD11b) aX (CD11c). also enhanced Toll‑like receptors (TLRs; TLR1, TLR2, TLR3 TLR4) triggered such as tumor necrosis factor α, interleukin (IL)‑1β, IL‑8 IL‑12. Proteomics showed higher activity levels TLR2 TLR4, along their downstream signaling adaptors myeloid differentiation primary response protein 88 (MyD88) Mal/TIR‑domain‑containing adapter (TIRAP), following treatment. Additionally, treatment rate cells. findings indicated regulate an vitro aseptic model activating TLR/TIRAP/MyD88 pathway.