N‑Myc downstream‑regulated gene 2 restrains glycolysis and glutaminolysis in clear cell renal cell carcinoma

• DOI: 10.3892/ol.2017.7024
• Publication Date: 2017-09-22T06:04:44Z
• Authors: Wei Shi; Xinyuan Xu; Fei Yan; Bao Wang; Hang Zhao; Aaron Chan; Zhen Ren; Yongzheng Ma; Fuli Wang; Jianlin Yuan
• Keywords: Glutaminolysis; Glutaminase; Anaerobic glycolysis

Glycolysis and glutaminolysis are heavily involved in the metabolic reprogramming of cancer cells. The activation oncogenes inactivation tumor suppressor genes has a marked effect on cellular processes glycolysis glutaminolysis. N‑Myc downstream‑regulated gene 2 (NDRG2) is that previous studies have demonstrated can inhibit growth, proliferation metastasis clear cell renal carcinoma (ccRCC) However, function NDRG2 ccRCC metabolism remains unknown. In present study, significantly inhibited consumption glucose glutamine, as well production lactate glutamate ccRCC. suppressed expression transporter 1, hexokinase 2, pyruvate kinase M2, dehydrogenase A, glutamine ASC amino acid glutaminase 1 at mRNA (by quantitative polymerase chain reaction) protein level western blot analysis), all which key regulators enzymes Data from study also revealed overexpression vitro vivo, by colony formation assays, wound healing assay nude mouse transplantation experiment. findings demonstrate for first time acts inhibitor could be promising target treatment