Curcumin inhibits the growth of liver cancer by impairing myeloid‑derived suppressor cells in murine tumor tissues

• DOI: 10.3892/ol.2021.12547
• Publication Date: 2021-02-12T18:09:42Z
• Authors: Sha Tian; Liu Liao; Qing Zhou; Xiaodi Huang; Piao Zheng; Yinmei Guo; Tianhao Deng; Xuefei Tian
• Keywords: Myeloid-derived Suppressor Cell

Curcumin, one of the active ingredients <em>Curcuma longa</em> (Jianghuang), has been reported to exert multiple bioactivities, including pro‑apoptotic and anti‑inflammatory activities. In recent years, curcumin extensively studied, it revealed that inhibits growth numerous types cancer. However, best our knowledge, inhibitory effects on activation or expansion myeloid‑derived suppressor cells (MDSCs) in liver cancer underlying mechanism have not yet determined. Therefore, present study aimed investigate effect MDSC activity associated anti‑neoplastic a HepG2 xenograft mouse model. The viability Huh‑7, MHCC‑97H <em>in vitro</em> was analyzed using Cell Counting Kit‑8 assay. tumor growth, numbers MDSCs, expression levels proteins involved toll‑like receptor 4 (TLR4)/NF‑κB signaling pathway, related inflammatory factors angiogenesis were determined model mice, which given different doses via intragastrical administration. results inhibited tumors mice. Flow cytometric analysis indicated reduced number MDSCs tumors. addition, demonstrated TLR4/NF‑κB pathway factors, IL‑6, IL‑1β, prostaglandin E2 cyclooxygenase‑2, Furthermore, suppressed secretion granulocyte‑macrophage colony‑stimulating factor (GM‑CSF) granulocyte‑colony stimulating (G‑CSF), are essential for modulation, tissues. Additionally, inhibit angiogenesis, by downregulation vascular endothelial factor, CD31 α‑smooth muscle actin western blotting, immunohistochemistry immunofluorescence experiments. conclusion, findings identified novel may suppress reducing subsequently disrupting process angiogenesis. These conclusions supported observed inactivation pathway‑mediated response GM‑CSF G‑CSF