ERC/mesothelin is expressed in human gastric cancer tissues and cell lines

• DOI: 10.3892/or.2013.2803
• Publication Date: 2013-10-22T10:37:56Z
• Authors: TOMOAKI ITO; KAZUNORI KAJINO; MASAAKI ABE; KOICHI SATO; HIROSHI MAEKAWA; MUTSUMI SAKURADA; HAJIME ORITA; RYO WADA; YOSHIAKI KAJIYAMA; OKIO HINO
• Keywords: Mesothelin

ERC/mesothelin is expressed in mesothelioma and other malignancies. The gene (MSLN) encodes a 71-kDa precursor protein, which cleaved to yield 31-kDa N-terminal (N-ERC/mesothelin) 40-kDa C-terminal (C-ERC/mesothelin) proteins. N-ERC/mesothelin soluble protein has been reported be diagnostic serum marker of ovarian cancer. Gastric cancer tissue also expresses C-ERC/mesothelin, but the significance N-ERC levels for diagnosing gastric not yet studied. We examined latter issue present study as well C-ERC/mesothelin expression human tissues cell lines. immunohistochemically samples from 50 cases cancer, we assessed 6 lines (MKN-1, MKN-7, MKN-74, NUGC-3, NUGC-4 TMK-1) using reverse transcription-polymerase chain reaction, flow cytometry, immunohistochemistry immunoblotting. concentrations supernatants cultured cells sera patients an enzyme-linked immunosorbent assay (ELISA). was detected 3 by ELISA, its concentration almost same that observed normal controls. In tissues, associated with lymphatic invasion. secreted into lines, does appear useful