MicroRNA miR-93-5p regulates expression of IL-8 and VEGF in neuroblastoma SK-N-AS cells

• DOI: 10.3892/or.2016.4676
• Publication Date: 2016-03-15T08:23:14Z
• Authors: ENRICA FABBRI; GIULIA MONTAGNER; NICOLETTA BIANCHI; ALESSIA FINOTTI; MONICA BORGATTI; ILARIA LAMPRONTI; GIULIO CABRINI; ROBERTO GAMBARI
• Keywords: Vascular Endothelial Growth Factor A; 0303 health sciences; Binding Sites; Base Sequence; Interleukin-8; Inverted Repeat Sequences; Gene Expression Regulation, Neoplastic; MicroRNAs; Neuroblastoma; 03 medical and health sciences; neuroblastoma, vascular endothelial growth factor (VEGF), interleukin-8 (IL-8); Cell Line, Tumor; microRNA, interleukin-8, vascular endothelial growth factor, neuroblastoma; Humans; Nucleic Acid Conformation; RNA Interference; 3' Untranslated Regions

The role of the microRNA miR-93-5p on the secretome profile and the expression levels of vascular endothelial growth factor (VEGF) and interleukin-8 (IL-8) was investigated in the neuroblastoma SK-N-AS cell line by Bio-Plex analysis and RT-qPCR. The results indicate that VEGF and IL-8 are the major miR-93-5p molecular targets. This conclusion was based on in vitro transfection with pre-miR-93-5p and anti-miR-93-5p; these treatments inversely modulated both VEGF and IL-8 gene expression and protein release in the neuroblastoma SK-N-AS cell line. Computational analysis showed the presence of miR-93-5p consensus sequences in the 3'UTR region of both VEGF and IL-8 mRNAs, predicting possible interaction with miR-93-5p and confirming a potential regulatory role of this microRNA.