High mobility group box 1 induces bone pain associated with bone invasion in a mouse model of advanced head and neck cancer

• DOI: 10.3892/or.2020.7788
• Publication Date: 2020-10-02T07:19:15Z
• Authors: Tomoya Nakamura; Tatsuo Okui; Kazuaki Hasegawa; Shoji Ryumon; Soichiro Ibaragi; Kisho Ono; Yuki Kunisada; Kyoichi Obata; Masanori Masui; Tsuyoshi Shimo; Akira Sasaki
• Keywords: HMGB1

Advanced head and neck cancer (HNC) can invade facial bone cause pain, thus posing a significant challenge to the quality of life patients presenting with advanced HNC. The present study was designed investigate HNC pain (HNC‑BP) in an intratibial mouse xenograft model that utilized cell line (SAS cells). These mice develop HNC‑BP is associated expression phosphorylated ERK1/2 (pERK1/2), which molecular indicator neuron excitation dorsal root ganglia (DRG) sensory neurons. Our experiments demonstrated inhibition high mobility group box 1 (HMGB1) by short hairpin (shRNA) transduction, HMGB1 neutralizing antibody, receptor antagonist suppressed pERK1/2 DRG. It also observed HNC‑derived increased acid‑sensing nociceptor, transient potential vanilloid (TRPV1), major osteoclastic Collectively, our results originating evokes via direct signaling hypersensitization for acid environment