Aberrant methylation‑mediated decrease of lncRNA HNF1A‑AS1 contributes to malignant progression of laryngeal squamous cell carcinoma via EMT

• DOI: 10.3892/or.2020.7823
• Publication Date: 2020-10-23T09:21:21Z
• Authors: Yewen Shi; Qingqing Zhang; Meng Xie; Yani Feng; Sijing Ma; Chunxi Yi; Zihan Wang; Yanju Li; Xiaohong Liu; Haiqin Liu; Hui Yang; Yan Yan; Yitong Zhang; Xiaoyong Ren; Huanan Luo
• Keywords: Male; 0301 basic medicine; Epithelial-Mesenchymal Transition; Down-Regulation; Laryngectomy; Articles; DNA Methylation; Epigenesis, Genetic; 3. Good health; Gene Expression Regulation, Neoplastic; 03 medical and health sciences; Chemotherapy, Adjuvant; Cell Line, Tumor; Gene Knockdown Techniques; Lymphatic Metastasis; Azacitidine; Disease Progression; Animals; Humans; CpG Islands; Female; Larynx; Laryngeal Neoplasms; Aged

Aberrant methylation is one of the most frequent epigenetic alterations that regulate expression levels genes, including long non‑coding RNAs (lncRNAs), in tumors. However, to best our knowledge, and function hepatic nuclear factor 1α antisense RNA 1 (HNF1A‑AS1) its condition have not yet been reported development progression laryngeal squamous cell carcinoma (LSCC). In present study, HNF1A‑AS1 were first examined by reverse transcription‑quantitative PCR, bisulfite genomic sequencing methylation‑specific polymerase chain reaction samples from patients with LSCC, which based on silico analysis using The Cancer Genome Atlas data, then further verified LSCC lines without 5‑Aza‑2'‑deoxycytidine (5‑Aza‑dC) treatment. Subsequently, proliferation, cycle distribution, migration invasion cells following either knockdown or overexpression determined vitro. Furthermore, characteristic epithelial‑mesenchymal transition (EMT) changes was investigated vitro vivo. associations between tumorigenicity cervical lymph node metastasis assessed a xenograft model nude mice. downregulation hypermethylation CpG sites detected tissues as well metastatic nodes when compared those adjacent non‑tumor tissues. Additionally, inhibited regulating process EMT. tumor growth EMT abilities, markers significantly reversed treatment 5‑Aza‑dC. summary, downregulated cancer cells. These findings suggested could serve suppressor lncRNA process, leading discovery novel therapeutic targets strategies for LSCC.