<b>Plant regeneration and histological study of the somatic embryogenesis of sugarcane (<i>Saccharum</i> spp.) cultivars RB855156 and RB72454</b> - doi: 10.4025/actasciagron.v36i1.16342

• DOI: 10.4025/actasciagron.v36i1.16342
• Publication Date: 2014-02-27T11:27:05Z
• Authors: Giovana Bomfim de...; Roberson Dibax; João Carlos Bespa...; Edelclaiton Daros
• Keywords: Callus; Saccharum

The development of an efficient regeneration system is crucial for the genetic transformation sugarcane. aims this study were to regenerate plants from somatic embryos and analyze origin division pattern cells during different embryonic developmental stages sugarcane cultivars RB855156 RB72454. For both cultivars, best results embryogenic callus induction obtained with explant cultures on culture medium containing 13.5 µM 2,4-dichlorophenoxyacetic acid (2,4-D) followed by solid 4.5 2,4-D. A higher rate shoot was observed in addition 8.9 17.8 benzylaminopurine (BAP); also RB72454 BAP. Murashige Skoog (1962) (MS) 2.5 5.0 indolebutyric (IBA) RB72454, respectively, suitable rooting. plantlets successfully acclimatized plant survival 100% cultivars. Histological analysis revealed that shoots originated embryogenesis