<b>Population genetics of the sugarcane borer <i>Diatraea saccharalis</i> (Fabr.) (Lepidoptera: Crambidae)

Crambidae Diatraea saccharalis
DOI: 10.4025/actasciagron.v36i2.16211 Publication Date: 2014-05-20T07:55:07Z
ABSTRACT
Diatraea saccharalis is the principal pest of sugarcane in Brazil and found throughout crop.Information about its population genetics scarce, but genetic analysis particular importance as a basis for successful control program.Pest requires constant evaluation variability so that appropriate strategies can be employed.In this study, structure D. populations crops was analyzed with PCR-RAPD (Polymerase Chain Reaction-Random Amplified Polymorphic DNA) markers.Samples were collected from four fields ParanáState one São PauloState.Twelve primers used.The produced 216 fragments, 99.54% polymorphism rate.The Shannon index averaged 0.3797 (± 0.1729), average value G ST five 0.0909.These results showed are differentiated.An molecular variance 92% occurs within populations.The high rate these shows insects have potential to develop resistance insecticides.The management should conducted cautiously integrated using chemical biological control.
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