Isolation and Genetic Characterization of Phenol-Degradating Bacterium from a Salt Lake in Inner Mongolia

• DOI: 10.4028/www.scientific.net/amr.726-731.396
• Publication Date: 2013-08-16T07:48:58Z
• Authors: Gang Dai; La Mei Liu; Qing Chao Ouyang; Chao Luo Meng; Tat Suo Iwasa
• Keywords: Catechol; Strain (injury); Dioxygenase

Salt lake environments are economically and ecologically important issues. Like any other ecosystems in the world, salt facing various kinds of pollutions. However, little information is available on biodegradation organic pollutants by halophilic microorganisms such environments. In present work we isolated a strain phenol-degradating bacteria from Inner Mongolia. Sequence 16S rRNA suggested that belong to Propionibacterium genus. The can grow with phenol as sole carbon source under 10% (w/v) concentration. PCR amplifications primers for multicomponent hydroxylase or catechol 1, 2 dioxygenase gene were applied genome DNA strain, suggesting might be transformed further degradated through ortho-ring cleavage pathway. When obtained water, at least two types genes sequences obtained, presence diverse aromatic compound-degradating genes.