Study on Fluorescent Staining of Bacteria for Total Direct Counts in Polycyclic Aromatic Hydrocarbons (PAHs) Contaminated Soil with 5-(4,6-DichloroTriazin-2-yl) Amino Fluorescein (DTAF)

• DOI: 10.4028/www.scientific.net/amr.726-731.452
• Publication Date: 2013-08-16T07:48:58Z
• Authors: Dan Zhang; Ai Zhong Ding; Lin Jiang; Yue Hua Cai; Li Na Zhang
• Keywords: 0303 health sciences; 03 medical and health sciences

Using DTAF fluorescent staining technique, the number of bacteria and the distributions of ΣPAHs in two different drilling vertical depths of a field contaminated by PAHs are counted and analyzed. The result showed that the distributions of the number of bacteria and the ΣPAHs in the vertical depth have obvious corresponding relations, and the higher the ΣPAHs is, the more the number of bacteria would be.