Data Mining of Nicotine Demethylase Genes in Modern Tobacoo

• DOI: 10.4028/www.scientific.net/amr.787.356
• Publication Date: 2013-09-04T12:35:13Z
• Authors: Jie Lian; De Ji Wang; Jun Ming Xie
• Keywords: Nornicotine; Demethylase

The predominant pyridine alkaloid in modern tobacco ( Nicotiana tabacum ) is Nicotine. However, with the existence of a class Nicotine demethylases, large proportion will be converted into Nornicotine during leaf senescence. Identification demethylases important, since nitrosated N'-nitrosonornicotine (NNN), which exhibit carcinogenic properties laboratory animals and harmful for human heath. Although many efforts have been performed to identified, no comprehensive analysis was carried out subfamily demethylases. In this work, we phylogenetic comparative their highly homologs. A single conserved mutation W->C prohibit activity Thus, could dramatically decreased if all demethylase were mutated by transgenic manipulation. Our results provide an insightful solid foundation further experimental design.