Characterization of the Effects of Endotoxin on Macrophage Tumor Cell Killing

• DOI: 10.4049/jimmunol.121.1.72
• Publication Date: 2022-12-30T23:03:58Z
• Authors: J Brice Weinberg; Harold A Chapman; John B Hibbs
• Keywords: Lipid A; Polymyxin B; Macrophage-activating factor

Abstract These studies demonstrate the potent effect of LPS on induction macrophage-mediated tumor cell killing and show, by using pure populations cloned macrophages as effector cells, that can act in complete absence contaminating lymphocytes. Macrophage differentiation toward tumoricidal state parallels responsiveness to LPS. Normal will not kill even presence 10 µg/ml LPS; peptone-induced normal (stimulated macrophages) are made ≥500 mg/ml nontumoricidal BCG-activated cells 0.5 1.0 ng/ml The is reproduced lipid A. abolished alkaline treatment or polymyxin B. Nontumoricidal from A-nonresponder mouse strain C3H/HeJ require 250 times more become than do C3H/HeN strain. acts synergistically with MAF-rich peritoneal supernatants causing cells. Although elicit MAF production lymphocytes, it also directly lymphocyte-free macrophage colonies render them tumoricidal. Reagents frequently contaminated LPS, unless this noted, valid interpretation experiments investigating difficult. Means detecting, detoxifying, eliminating experimental agents discussed.