Role of interleukin 1 in promoting human monocyte-mediated tumor cytotoxicity.

• DOI: 10.4049/jimmunol.135.1.314
• Publication Date: 2023-01-02T19:05:04Z
• Authors: K Onozaki; K Matsushima; E S Kleinerman; T Saito; J J Oppenheim
• Keywords: Monocyte; Interleukin 19

Human peripheral blood monocytes from normal donors obtained by separation on a Percoll gradient showed considerable cytotoxicity against tumor cells when preincubated in vitro for 24 hr with human monocyte-derived interleukin 1 (IL 1). In contrast, after pretreatment medium alone had low cytotoxic activity. All the IL preparations, including which was purified high-performance liquid column chromatography (HPLC), as well crude culture supernatant promoted monocyte-mediated same dose-dependent manner thymocyte growth-promoting There no endotoxin or interferon (IFN) activity highly 1, suggesting that itself active moiety. The effect of partially inhibited indomethacin, whereas prostaglandin (PG) E1 E2 rather than also resulted substantial monocyte cytotoxicity. Thus, is presumably mediated PGE. Since fresh were not exhibited levels spontaneous similar to it seemed likely maintain level induce To elucidate this possibility, first longer period, and losing their they further incubated without 1. Such "aged" did develop response but other agents known macrophage cytotoxicity, such lymphokine-containing supernatants. Therefore, major actually prolong state monocytes. These results suggest released macrophages may play role host defense neoplastic acting an autostimulating factor.