The effects of N-3 polyunsaturated fatty acids on the generation of platelet-activating factor-acether by human monocytes.

• DOI: 10.4049/jimmunol.139.12.4186
• Publication Date: 2022-12-31T03:35:49Z
• Authors: R I Sperling; J L Robin; K A Kylander; T H Lee; R A Lewis; K F Austen
• Keywords: Platelet-activating factor; Monocyte

Human leukocytes generate platelet-activating factor (PAF-acether), a lipid mediator of inflammation, from membrane alkyl phospholipids through the release arachidonic acid or other fatty acids at 2-position and subsequent acetylation. Because it was previously demonstrated that fish oil suppress human leukocyte metabolism, separate experiments were conducted to investigate effects dietary supplementation in vitro incubation with on calcium ionophore-stimulated PAF-acether generation monocytes. In subjects their regular diets, 4-hr monocyte monolayers an optimally effective concentration 1 micrograms/ml resulted 64% increase ionophore-induced net 7.75 +/- 0.78 ng/10(6) cells for untreated 12.70 1.21 (mean SEM). Treatment eicosapentaenoic (EPA) optimal decreased by 28%. However, treatment docosahexaenoic did not appreciably affect generation. The changes each added paralleled those total generation; percentage remained unaffected. Three weeks 18 g MaxEPA daily, providing 3.2 EPA monolayers. 6 47% decrease concomitant 59% decline release; affected. Thus, whether diet introduced vitro, oil-derived