CD28-stimulated IL-2 gene expression in jurkat T cells occurs in part transcriptionally and is cyclosporine-A sensitive

• DOI: 10.4049/jimmunol.149.2.745.c
• Publication Date: 2023-01-01T00:22:02Z
• Authors: TM Williams; DM Moolten; H Makni; HW Kim; JA Kant; M Kamoun
• Keywords: Jurkat cells

CD28 is a glycoprotein expressed as homodimer on the surface of major subset human T cells. Previous studies have shown that proliferation peripheral blood cells involving pathway associated with cyclosporine A (CsA) resistant IL-2 gene expression. This was to specifically regulate stability mRNA for several lymphokines including IL-2. We investigated expression in Jurkat cell line, J32 clone, induced by stimulation. Cross-linked anti-CD28 mAb alone sufficient induce release small amounts (256 U/ml). The CD28-mediated enhanced simultaneous engagement and CD2 or CD3 molecules. Hybrid constructs which 5' flanking region drives luciferase (pIL-2-Luc) were used help delineate whether activates transcriptionally. Costimulation either PHA 20- 90-fold increase pIL-2-Luc well release. plus PMA gave only five- sevenfold enhancer activity. In contrast, no activity detected after stimulation alone. Both inhibited CsA degree inhibition concentration dependent similar stimulated mAb. Maximum achieved 1 microgram/ml CsA. Studies internal deletion mutations sequence revealed through TCR pathway, requires sequences located within 500 bp transcription start site. These results are first direct evidence triggering molecule Furthermore these strongly indicate occurs, part, transcriptionally sensitive