Transcriptional activation of the human TNF-α promoter by superantigen in human monocytic cells: role of NF-kappa B

• DOI: 10.4049/jimmunol.155.2.902
• Publication Date: 2023-01-01T00:19:31Z
• Authors: N S Trede; A V Tsytsykova; T Chatila; A E Goldfeld; R S Geha
• Keywords: Kappa; Superantigen

We have studied the transcriptional activation of human TNF-alpha gene by superantigen staphylococcal enterotoxin A (SEA) in premonocytic cell line THP-1. Nuclear proteins from SEA-stimulated THP-1 cells bound strongly to kappa 3, most proximal three putative NF-kappa B binding sites (kappa 1-kappa 3) found 5' regulatory region gene, but only weakly 1, distal sites, and showed no 2. The mobility 3-nucleoprotein complex was identical that complexes formed between nuclear consensus seuqence. Moreover, both 3' mutants 3 were unable displace binding, suggesting induced SEA has characteristics B. Studies using Abs directed against subunits p50 p65 suggested bind sequence. Reporter assays deletion (-99 -89 bp) point mutation guanine bases sequence reduced inducibility promoter LPS. These results indicate induces monocytic suggest site plays an important role superantigen.