Patterns of Chemokine Receptor Expression on Peripheral Blood γδ T Lymphocytes: Strong Expression of CCR5 Is a Selective Feature of Vδ2/Vγ9 γδ T Cells

• DOI: 10.4049/jimmunol.168.10.4920
• Publication Date: 2014-04-20T22:26:04Z
• Authors: Andrea Glatzel; Daniela Wesch; Florian Schiemann; Ernst Brandt; Ottmar Janssen; Dieter Kabelitz
• Keywords: CCL5; CCR1; Chemokine receptor CCR5; CC chemokine receptors; CXCL16; CCL13; CCL17

Abstract γδ T lymphocytes play an important role in the immune defense against infection, based on unique reactivity of human Vδ2Vγ9 cells toward bacterial phosphoantigens. Chemokines and their corresponding receptors orchestrate numerous cellular reactions, including leukocyte migration, activation, degranulation. In this study we investigated expression various for inflammatory homeostatic chemokines peripheral blood compared patterns with those αβ cells. Although several analyzed (including CCR6, CCR7, CXCR4, CXCR5) were not differentially expressed vs cells, strongly increased levels RANTES/macrophage protein-1α/-1β receptor CCR5 also enhanced CCR1–3 CXCR1–3. was restricted to Vδ2 while minor subset Vδ1 preferentially CXCR1. Stimulation heat-killed extracts Mycobacterium tuberculosis down-modulated cell surface a macrophage-dependent manner, synthetic phosphoantigen isopentenyl pyrophosphate ligands directly triggered down-modulation The functionality chemokine CXCR3 demonstrated by Ca2+ mobilization chemotactic response respective chemokines. Our results identify high level as characteristic selective feature circulating which is line suspected function Th1 effector