Cationic Polypeptides Are Required for Antibacterial Activity of Human Airway Fluid

• DOI: 10.4049/jimmunol.169.12.6985
• Publication Date: 2014-04-21T00:33:07Z
• Authors: Alexander M Cole; Hsiang-I Liao; Olga Stuchlik; Jason Tilan; Jan Pohl; Tomas Ganz
• Keywords: Cationic polymerization; Lactoferrin

In a search for direct evidence leading to the biological relevance of airway secretions in innate host defense, we characterized antibacterial function cationic polypeptides within minimally manipulated nasal fluid. this study, show that antimicrobial are responsible most bactericidal activity whole The removal using cation-exchange resin ablated fluid against Escherichia coli, Listeria monocytogenes, and Pseudomonas aeruginosa. By novel proteomic approach, identified dozen peptides proteins fluid, all which either known or have other proposed roles defense. Of three abundant lysozyme was more effective than lactoferrin secretory leukoprotease inhibitor restoring polypeptide-depleted mucoid cystic fibrosis isolate P.