Regulated Expression of the Receptor-Like Tyrosine Phosphatase CD148 on Hemopoietic Cells

• DOI: 10.4049/jimmunol.173.4.2324
• Publication Date: 2014-04-22T04:58:37Z
• Authors: Joseph Lin; Jing W Zhu; Jeanne E Baker; Arthur Weiss
• Keywords: B-Lymphocytes; Immunity, Cellular; T-Lymphocytes; Cell Differentiation; Hematopoietic Stem Cells; Lymphocyte Activation; 03 medical and health sciences; 0302 clinical medicine; Cricetinae; Leukocytes; Animals; Cell Lineage; Enzyme Inhibitors; Protein Tyrosine Phosphatases

Abstract CD148 is a receptor-like protein tyrosine phosphatase expressed on wide variety of cell types. Through the use flow cytometry and immunofluorescence microscopy tissue sections, we examined expression multiple murine hemopoietic lineages. We found that moderately during all stages B development in bone marrow, as well peripheral mature cells. In contrast, thymocytes T cells substantially lower. However, stimulation through TCR leads to an increase expression. This up-regulation can be partially inhibited by reagents block activity src family kinases, calcineurin, MEK, or PI3K. Interestingly, levels are elevated freshly isolated from MRL lpr/lpr CTLA-4-deficient mice, two models autoimmunity. Together, these data along with previous biochemical suggest may play important regulatory role control immune response.