Targeting of Antigen to Dendritic Cells with Poly(γ-Glutamic Acid) Nanoparticles Induces Antigen-Specific Humoral and Cellular Immunity

• DOI: 10.4049/jimmunol.178.5.2979
• Publication Date: 2014-04-18T17:14:39Z
• Authors: Tomofumi Uto; Xin Wang; Katsuaki Sato; Misako Haraguchi; Takami Akagi; Mitsuru Akashi; Masanori Baba
• Keywords: Splenocyte

Abstract Nanoparticles are considered to be efficient tools for inducing potent immune responses by an Ag carrier. In this study, we examined the effect of Ag-carrying biodegradable poly(γ-glutamic acid) (γ-PGA) nanoparticles (NPs) on induction in mice. The NPs were efficiently taken up dendritic cells (DCs) and subsequently localized lysosomal compartments. γ-PGA strongly induced cytokine production, up-regulation costimulatory molecules, enhancement T cell stimulatory capacity DCs. These maturational changes DCs involved MyD88-mediated NF-κB signaling pathway. vivo, preferentially internalized APCs (DCs macrophages) production IL-12p40 IL-6. immunization mice with OVA-carrying Ag-specific CTL activity IFN-γ splenocytes as well IgG1 IgG2a Abs serum. Furthermore, carrying a CD8+ epitope peptide Listeria monocytogenes significantly protected infected from death. results suggest that capable strong cellular humoral might potentially useful effective vaccine adjuvants therapy infectious diseases.