A Role of Toll-IL-1 Receptor Domain-Containing Adaptor-Inducing IFN-β in the Host Response to Pseudomonas aeruginosa Lung Infection in Mice

• DOI: 10.4049/jimmunol.178.5.3170
• Publication Date: 2014-04-18T17:14:39Z
• Authors: Melanie R. Power; Bo Li; Masahiro Yamamoto; Shizuo Akira; Tong-Jun Lin
• Keywords: TRIF; CXCL1

Abstract Toll-IL-1R domain-containing adaptor-inducing IFN-β (TRIF) is an adaptor molecule that mediates a distinct TLR signaling pathway. Roles of TRIF in the host defense have been primarily associated with virus infections owing to induction IFN-αβ. In this study, we investigated role Pseudomonas aeruginosa infection. vitro, TRIF-deficient mouse alveolar and peritoneal macrophages showed complete inhibition RANTES (CCL5) production, severely impaired TNF KC (CXCL1) reduced NF-κB activation response P. stimulation. vivo, mice efficient MIP-2 IL-1β production lung following This outcome was delayed recruitment neutrophils into airways. These results suggest cytokine/chemokine profile aeruginosa-induced completely dependent on pathway mice. Importantly, deficiency leads clearance from during initial 24–48 h Thus, represents novel mechanism involved development