Dynamic Relationship between IFN-γ and IL-2 Profile of Mycobacterium tuberculosis-Specific T Cells and Antigen Load

• DOI: 10.4049/jimmunol.178.8.5217
• Publication Date: 2014-04-18T22:55:39Z
• Authors: Kerry A. Millington; John A. Innes; Sarah Hackforth; Timothy S. C. Hinks; Jonathan J. Deeks; Davinder P. S. Do...; Valerie Guyot-Revol; Rubamalaar Gunath...; Paul Klenerman; Ajit Lalvani
• Keywords: Adult; Aged, 80 and over; Male; Antigens, Bacterial; Adolescent; T-Lymphocytes; 610; Mycobacterium tuberculosis; Middle Aged; 3. Good health; Interferon-gamma; 03 medical and health sciences; 0302 clinical medicine; Bacterial Proteins; 616; Humans; Interleukin-2; Female; Aged; Follow-Up Studies

Abstract Distinct IFN-γ and IL-2 profiles of Ag-specific CD4+ T cells have recently been associated with different clinical disease states Ag loads in viral infections. We assessed the kinetics functional profile Mycobacterium tuberculosis secreting 23 patients untreated active when bacterial are high after curative treatment, load is reduced. The frequencies M. IFN-γ-secreting declined during 28 mo follow-up an average percentage decline 5.8% per year (p = 0.005), while IL-2-secreting increased treatment 0.02). These contrasting dynamics for two cytokines led to a progressive convergence IFN-γ- over mo. Simultaneous measurement secretion at single-cell level revealed codominance IFN-γ-only IFN-γ/IL-2 dual that shifted dominance IFN-γ/IL-2-secreting newly detectable IL-2-only treatment. distinct cell signatures before suggest novel immunological marker mycobacterial status now requires validation larger prospective studies.