CD45 Regulates TLR-Induced Proinflammatory Cytokine and IFN-β Secretion in Dendritic Cells

• DOI: 10.4049/jimmunol.180.12.8020
• Publication Date: 2014-04-18T23:24:59Z
• Authors: Jennifer L Cross; Katharine Kott; Tatjana Miletić; Pauline Johnson
• Keywords: Proinflammatory cytokine

Abstract CD45 is a leukocyte-specific protein tyrosine phosphatase and an important regulator of AgR signaling in lymphocytes. However, its function other leukocytes not well-understood. In this study, we examine the dendritic cells (DCs). Analysis DCs from CD45-positive CD45-null mice revealed that required for development but does influence DC maturation induced by TLR agonists. affected phosphorylation state Lyn, Hck, Fyn bone marrow-derived dysregulated LPS-induced Lyn activation. TLR4-induced proinflammatory cytokine IFN-β secretion TLR4-activated had reduced ability to activate NK Th1 produce IFN-γ. Interestingly, effect on TLR-induced depended activated. CD45-negative indicated negative TLR2 9, MyD88-dependent production, positive TLR3 4, MyD88-independent secretion. This indicates new role regulating responses implicates wider regulatory innate adaptive immunity.