MicroRNA miR-146a is a critical suppressor of arthritis in the mouse model of Lyme Disease (P1275)

• DOI: 10.4049/jimmunol.190.supp.56.20
• Publication Date: 2023-01-01T09:43:17Z
• Authors: Robert Lochhead; Ryan O’Connell; Ying Ma; James Zachary; John Weis; Janis Weis
• Keywords: 0301 basic medicine; 03 medical and health sciences; 3. Good health

Abstract Lyme Disease is caused by infection with the spirochete Borrelia burgdorferi, and most common vector-borne disease in United States. Often, leads to acute arthritis humans. Our lab others have exploited mouse model system elucidate mechanical mechanisms of development. Because miRNAs been shown be important regulators inflammatory immune responses, we performed a microarray screen for expression joint tissue from three infected strains. This identified upregulation miR-146a, key negative regulator NF-κB signaling. Others previously demonstrated that Toll-like receptor 2/MyD88 dependent activation major response mice humans, but determining its role development has remained elusive. We show miR-146a plays critical nonreduntant modulating Borrelia-induced NF- κB infection, lacking this miRNA develop more severe without compromising response. Furthermore, miR-146a-/- elevated transcript levels NF-κB-regulated cytokines chemokines tissue, as well increased myeloid cell infiltration, linking dysregulation Together, these data required suppression Arthritis, provides much-needed studying