IL-6 regulates pro- and anti-inflammatory cytokine production and mortality of Mycobacterium tuberculosis infected type 2 diabetic mice (INC2P.418)

• DOI: 10.4049/jimmunol.194.supp.55.12
• Publication Date: 2023-01-01T10:08:22Z
• Authors: Satyanarayana Che...; Deepak Tripathi; Sambasivan Venkat...; Padmaja Paidipally; Elwyn Welch; Amy Tvinnereim; Hardy Kornfeld; Ramakrishna Vanka...
• Keywords: 0303 health sciences; 03 medical and health sciences; 3. Good health

Abstract In this study, to determine whether diabetic (DM) mice are susceptible Mycobacterium tuberculosis (Mtb) infection, we developed experimentally induced diabetes in wild type C57BL/6 with streptozotocin & nicotinamide. We then infected non-DM and DM Mtb H37Rv by aerosol. Around 50% of (p=0.05) 25% uninfected (p=0.07) died 5 6 months compared no deaths control mice. Six after there was a statistically significant but marginal increase bacterial burden the lungs (3.1 ± 0.4 x106 vs 0.9 0.1 x 106, p=0.001) suggesting increased mortality not due burden. Real time PCR analysis indicated significantly pro- anti-inflammatory cytokine gene expression mice, including levels IL-6 mRNA. Anti-IL-6 antibody treatment enhanced survival (100% 60% isotype treated, p=0.02) reduced production. Dendritic cells (DC), other immune were major source for NK further production autologous DCs. Our results suggest NK-DC interaction enhance which drives pathology